Spoilage organisms and contamination present significant challenges to the brewing industry, making microbiological testing essential throughout the brewing process. However, traditional enrichment methods are slow, requiring 3 to 5 days before beer can be approved for market release. This delay increases product hold times and operational costs.
Rapid microbiological analysis offers a promising alternative, enabling faster spoilage detection. However, these methods often face limitations due to small analysis volumes.
This study explores the effectiveness of InnovaPrep’s Concentrating Pipette in concentrating spoilage organisms from 12 ounces (355mL) of beer and 750mL of wine into volumes suitable for rapid detection. Beer and wine samples were experimentally infected with Lactobacillus brevis and analyzed using qPCR.
Key Findings:
- The Concentrating Pipette achieved 70% to 92% concentration recovery efficiency in beer samples.
- In red and white wine, it concentrated L. brevis by 1000X to 2000X, significantly enhancing detection capabilities.
This innovative approach streamlines microbiological testing, reducing delays and improving contamination control in brewing and winemaking.
Download the Poster Concentration of Lactobacillus brevis from Experimentally Infected American Lager Beer and Wine by Innovaprep’s Concentrating Pipette and Be Flat Degassing Jar
