Using water samples from marine animal enclosures at the Shedd Aquarium, as well as artificial seawater, researchers intentionally introduced various bacteriophages and viruses into the samples to evaluate the efficiency of the Concentrating Pipette for marine applications.
The Concentrating Pipette effectively concentrated the organisms from the water matrix and concentrates were analyzed using digital droplet PCR (ddPCR). The efficiency rates varied, reaching up to 82%, with concentration factors ranging from 60 to 200-fold.
"In this study, we evaluated the performance of the Concentrating Pipette, a compact and automated and portable bio-concentration device for the recovery of viruses in marine water samples. This procedure allows for concentrating a variety of viruses in a rapid sample processing turnaround time. This procedure can also be implemented in other marine or coastal environments, such as recreational beaches for monitoring viruses to protect public health."
The simplicity of using the CP Select for concentration coupled with ddPCR for analysis facilitates routine monitoring of marine habitats for viruses, thereby proving useful for monitoring disease pathogens of raised fish for aquaculture, marine mammal health monitoring, and for public health at recreational beaches.
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Evaluation of an Automated Ultrafiltration System for Concentrating a Range of Viruses from Marine Waters – Singh et al. (preprint)
