Researchers at the University of Minnesota conducted a study using a sophisticated bioaerosol test chamber to compare eight bio samplers for their effectiveness in capturing airborne viruses. The focus was on rapid detection and preserving the infectivity of viruses. They tested MS2 bacteriophage (as a small surrogate), H3N2 avian influenza, and H9N9 swine influenza, which have implications for both human and animal health.
Samples were collected according to the manufacturers' instructions over a 30-minute period. The samples were then extracted using the provided kits and analyzed in parallel using PCR and TCID-50 assays. The SpinCon II and Bobcat Air Sampler were the most effective, with the Bobcat surprisingly capturing a high quantity of infectious H3N2 and H9N9.
"Portability and ease of use are important considerations in selecting any sampler. The Bobcat was the easiest of the eight samplers to use while also being easily portable."
The Bobcat demonstrated exceptional performance in sampling viral aerosols, making it a valuable tool for rapid detection and infectivity studies, which can enhance the efficiency and accuracy of analyses in animal and public health settings.
Read the article:
Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones Raynor et al. PLOS ONE 2021
