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Dealing with naturally occurring Interferents, Part 5

While the SCODA technique does have its limitations, it has been shown to be effective.  A quantitative evaluation of SCODA’s ability to reject humic acids, and a comparison to existing technologies was recently demonstrated at Stanford (Cold Spring Harbor Protocols).  Distilled water samples containing 2ng of DNA were spiked with increasing concentrations of humic acids.  The samples were then purified using a commercial silica column DNA purification kit, a commercial immuno bead-based isolation system, and  Boreal’s SCODA technologies. Continue…

Dealing with Interferents – Part 4

The SCODA system is a relatively new technique, but is well proven and is currently being coupled with the InnovaPrep process in the Department of Homeland Securities’ Automated Environmental Sample Preparation (AESaP) program.  InnovaPrep and Boreal Genomics are subcontractors to ICx Biosystems under the AESP program and coupling of the two systems prior to qPCR analysis has been demonstrated. Continue…

Posted Jan 4 2011 by dalburty with 0 Comments

Interferents Part 3

Some examples of demonstrated secondary clean-up methods that can be used subsequent to the InnovaPrep process include immunomagnetic separation (IMS), affinity techniques and electrophoretic techniques.  After using the InnovaPrep process to concentrate samples into volumes of 50 microliters to 1 mL, these clean-up methods can be more readily used and, as is the case with IMS, the techniques become more efficient.  Continue…

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